Journal: PLoS ONE

Article Title: 3-O-Galloylated Procyanidins from Rumex acetosa L. Inhibit the Attachment of Influenza A Virus

doi: 10.1371/journal.pone.0110089

Figure Lengend Snippet: IAV and test compounds were co-incubated for 1 h at 37°C prior to the addition to MDCK II cells. Heparin served as positive control (D). Values (% of plaque reduction) ±SD relate to the respective mock-treated controls ( = 100%). * p<0.05, ** p<0.01 (two-tailed, unpaired Student's t-test).

Article Snippet: MDCK II cells (canine kidney cells) were propagated in minimal essential medium (MEM; Biochrom, Berlin, Germany) supplemented with 5% fetal calf serum (FCS; Biochrom), 2 mM l -glutamine (Sigma-Aldrich), non-essential amino acids (1×) and 100 μg/mL penicillin/streptomycin (Biochrom).

Techniques: Incubation, Positive Control, Two Tailed Test

Journal: PLoS ONE

Article Title: 3-O-Galloylated Procyanidins from Rumex acetosa L. Inhibit the Attachment of Influenza A Virus

doi: 10.1371/journal.pone.0110089

Figure Lengend Snippet: Effects on the penetration of IAV were determined by a modified plaque reduction assay. Test compounds were added for 30 min. after attachment of IAV to MDCK II cells at 4°C. Values (% of plaque reduction) ±SD relate to the respective mock-treated controls ( = 100%) and represent ≥3 independent experiments. * p<0.05, ** p<0.01 (two-tailed, unpaired Student's t-test).

Article Snippet: MDCK II cells (canine kidney cells) were propagated in minimal essential medium (MEM; Biochrom, Berlin, Germany) supplemented with 5% fetal calf serum (FCS; Biochrom), 2 mM l -glutamine (Sigma-Aldrich), non-essential amino acids (1×) and 100 μg/mL penicillin/streptomycin (Biochrom).

Techniques: Modification, Two Tailed Test

Journal: ACS Omega

Article Title: Conducting Polymer Scaffolds Based on Poly(3,4-ethylenedioxythiophene) and Xanthan Gum for Live-Cell Monitoring

doi: 10.1021/acsomega.8b00458

Figure Lengend Snippet: MDCK II eGFP cell growth on PEDOT:xanthan gum (0.5:0.5) 2% solid content scaffold 14 days after culture, 37 °C, 5% CO 2 . (a) Confocal microscopy image of the entire scaffold 14 days after cell seeding and (b) zoomed image of the scaffold 14 days after cell seeding.

Article Snippet: MDCK II LifeAct cells were prepared for this study in the same manner according to the manufacturer guidelines (Ibidi, GmbH) and as described previously using pCMVLifeAct-TagRFP.

Techniques: Confocal Microscopy

Journal: ACS Omega

Article Title: Conducting Polymer Scaffolds Based on Poly(3,4-ethylenedioxythiophene) and Xanthan Gum for Live-Cell Monitoring

doi: 10.1021/acsomega.8b00458

Figure Lengend Snippet: Cell growth on PEDOT:xanthan gum (0.5:0.5) 2% solid content scaffolds and impedance measurements. (a) Device configuration of the scaffold-based electrode. (b) Confocal microscopy image of the entire scaffold 7 days after cell seeding with fluorescent MDCK II LifeAct cells and (c,d) zoomed images of the scaffold 7 days after cell seeding. Impedance spectra showing the (e) complex impedance data, (f) the phase angle vs frequency, and the (g) Nyquist plots of the scaffolds before and after cell culture for 7 days. The dashed lines represent the spectra of a PEDOT:xanthan gum scaffold after 7 days of incubation (37 °C, 5% CO 2 ) in culture media without cells.

Article Snippet: MDCK II LifeAct cells were prepared for this study in the same manner according to the manufacturer guidelines (Ibidi, GmbH) and as described previously using pCMVLifeAct-TagRFP.

Techniques: Confocal Microscopy, Cell Culture, Incubation

Journal: bioRxiv

Article Title: Epithelial polarity drives tissue tension in planar, free standing cell monolayers

doi: 10.1101/2025.03.06.641857

Figure Lengend Snippet: Fabrication of the small testing device. A) Scheme of the PLA (polylactic-acid) block containing two glass rods. B) Phase contrast image of MDCK II cells attached to the glass rods, forming a planar tissue. Scale bar: 1 mm. C) Picture of the device and the free-standing collagen matrix.

Article Snippet: Then 29,000 MDCK II cells in 4 μ L culture medium with 0.1% Penicillin/Streptomycin solution (Pen/Strep; Merck, Darmstadt, Germany) are prepared and seeded onto the collagen support of the testing mount, while for the grid mount 3 · 10 5 cells in 180 μ L were used, respectively.

Techniques: Blocking Assay

Journal: bioRxiv

Article Title: Epithelial polarity drives tissue tension in planar, free standing cell monolayers

doi: 10.1101/2025.03.06.641857

Figure Lengend Snippet: Preparation of free-standing cell monolayers on a grid device. A) Placement of the collagen at the level of the glass ring (I) and subsequent cell seeding (II). Treatment with collagenase to remove the matrix, resulting in a cell monolayer anchored at the edges of the glass without extra support (III). B) Confocal image of an immunostained MDCK II monolayer after removal of the collagen matrix. Anti-podocalyxin is used to label the apical domain (red), while anti-laminin highlights the basal domain (green). DAPI is used to stain the nuclei (blue). Note that the surface is uneven generating a gradient of the different layers of the cells. C) Orthogonal view of the immunostained MDCK II monolayer. Scale bar: 20 μm. D) Schematic illustration of the grid device, consisting of a glass ring where the cells attach basally, a metal grid to initially hold the collagen and the support of the structure. E) Orthogonal view of the device (cross section), showing how turning the device gives access to both sides, apical and basal.

Article Snippet: Then 29,000 MDCK II cells in 4 μ L culture medium with 0.1% Penicillin/Streptomycin solution (Pen/Strep; Merck, Darmstadt, Germany) are prepared and seeded onto the collagen support of the testing mount, while for the grid mount 3 · 10 5 cells in 180 μ L were used, respectively.

Techniques: Staining

Journal: bioRxiv

Article Title: Epithelial polarity drives tissue tension in planar, free standing cell monolayers

doi: 10.1101/2025.03.06.641857

Figure Lengend Snippet: Indentation response of the monolayer. A) Indentation-relaxation experiments (force-time curves) of the apical (left) and basal (right) side of a free-standing cell monolayer loaded up to different setpoints. The response of the basal domain is purely elastic, while the apical side shows slow force relaxation. B) Indentation-retraction experiments (force-height curves) of the apical (left) and basal (right) side of a cell monolayer up to a setpoint of 100 nN. C) Model of the indentation experiment. The yellow line represents the cell monolayer adopting a catenoid shape and the black circle denotes the indenter. R is the radius of the aperture covered by the monolayer (inner circle of the glass ring), z a is the indentation depth at r = a, R p the radius of the indenter, and a is the contact radius of the cell monolayer with the indenter. D) The force versus indentation (height) plot illustrates how the tissue tension is obtained as the slope of the curve. E) Tissue tension is derived from force curves with varying maximal indentation forces. At higher maximal forces (setpoints), cell monolayers loaded apically and basally exhibit identical tissue tension. F) Tissue tension as a function of drug administration with a following probing from the apical side of the cell monolayer. Crtl: MDCK II cell monolayer in the absence of drugs (control); Bleb: blebbistatin (50 μM, stalling myosin II motors); Caly: calyculin A (20 nM, inhibiting phosphatase); GDA: glutardialdehyde (fixation).

Article Snippet: Then 29,000 MDCK II cells in 4 μ L culture medium with 0.1% Penicillin/Streptomycin solution (Pen/Strep; Merck, Darmstadt, Germany) are prepared and seeded onto the collagen support of the testing mount, while for the grid mount 3 · 10 5 cells in 180 μ L were used, respectively.

Techniques: Derivative Assay, Control